bioGenousTM Mouse Colonic Organoid Kit is a serum-free culture medium designed for the primary culture and differentiation of mouse colonic organoids. During the primary culture phase, the organoids are primarily composed of colonic stem cells (LGR5+) and progenitor cells. Upon differentiation, the mouse colonic organoids also contain colonic absorptive cells (VILLI+) and goblet cells (MUC2+). These organoids faithfully replicate the characteristics of the in vivo colonic epithelium in terms of self-renewal and differentiation capabilities, tissue architecture, cell type composition, and functionality. In summary, bioGenousTM Mouse Colonic Organoid Kit provides an ideal in vitro model for studying mouse colon biology.